Data/hora: 17/06/2024 14:57:16

Provedor de dados: | 69
País: | Chile
Título: | Expression of the antibody 14D9 in Nicotiana tabacum hairy roots
Autores: | Martínez,Carolina; Petruccelli,Silvana; Giulietti,Ana María; Alvarez,María Alejandra.
Data: | 2005-08-01
Ano: | 2005
Palavras-chave: | Catalytic antibodies; In vitro cultures; Molecular farming; Recombinant antibodies; Tobacco.
Resumo: | Nicotiana tabacum hairy roots that express the antibody 14D9 were established. The 14D9 antibody yield obtained after 20 days of culture was 5.95 &amp;#956;g 14D9ml-1. The addition of the reticulum endoplasmic retention sequence KDEL demonstrated a positive effect over the intracellular 14D9 amounts with a yield increase up to 20.82 µg ml-1. DMSO increased the antibody amount in the biomass from 20.00 to 64.03 µg ml-1 while PVP (at 1.5 gl-1) and gelatine (at 5.0 gl-1) increased total 14D9 amounts in the culture medium to 25 µg and 14 µg respectively
Tipo: | Journal article
Idioma: | Inglês
Identificador: | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582005000200006
Editor: | Pontificia Universidad Católica de Valparaíso
Formato: | text/html
Fonte: | Electronic Journal of Biotechnology  v.8 n.2 2005