Data/hora: 11/06/2024 04:29:33

Provedor de dados: 	69	
País: 	Chile	
Título: 	Expression of the antibody 14D9 in Nicotiana tabacum hairy roots	
Autores: 	Martínez,Carolina; Petruccelli,Silvana; Giulietti,Ana María; Alvarez,María Alejandra.	
Data: 	2005-08-01	
Ano: 	2005	
Palavras-chave: 	Catalytic antibodies; In vitro cultures; Molecular farming; Recombinant antibodies; Tobacco.	
Resumo: 	Nicotiana tabacum hairy roots that express the antibody 14D9 were established. The 14D9 antibody yield obtained after 20 days of culture was 5.95 &amp;#956;g 14D9ml-1. The addition of the reticulum endoplasmic retention sequence KDEL demonstrated a positive effect over the intracellular 14D9 amounts with a yield increase up to 20.82 µg ml-1. DMSO increased the antibody amount in the biomass from 20.00 to 64.03 µg ml-1 while PVP (at 1.5 gl-1) and gelatine (at 5.0 gl-1) increased total 14D9 amounts in the culture medium to 25 µg and 14 µg respectively	
Tipo: 	Journal article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582005000200006	
Editor: 	Pontificia Universidad Católica de Valparaíso	
Formato: 	text/html	
Fonte: 	Electronic Journal of Biotechnology  v.8 n.2 2005