Data/hora: 09/06/2024 15:36:06

Provedor de dados: | Electronic Journal of Biotechnology
País: | Chile
Título: | Sodium azide mutagenesis resulted in a peanut plant with elevated oleate content
Autores: | Wang,Chuan Tang; Tang,Yue Yi; Wang,Xiu Zhen; Zhang,Shu Wei; Li,Gui Jie; Zhang,Jian Cheng; Yu,Shan Lin.
Data: | 2011-03-01
Ano: | 2011
Palavras-chave: | Chemical mutant; FAD2B; NIRS; Oleate; Peanut.
Resumo: | Screening of peanut seeds resulting from 0.39% sodium azide treatment with NIRS calibration equation for bulk seed samples identified a plant with more than 60% oleate. Oleate content in individual seeds of the plant, as predicted by NIRS calibration equation for intact single peanut seeds, ranged from 50.05% ~ 68.69%. Three seeds with &gt;60% oleate thus identified were further confirmed by gas chromatography. Multiple sequence alignments of the FAD2B gene from Huayu 22 (wild type) and peanut seeds with elevated oleate (mutant type) revealed a C281T transition in the coding region causing an I94T substitution in the oleoyl-PC desaturase, which may be responsible for reduction in the enzyme activity.
Tipo: | Journal article
Idioma: | Inglês
Identificador: | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000200010
Editor: | Pontificia Universidad Católica de Valparaíso
Formato: | text/html
Fonte: | Electronic Journal of Biotechnology  v.14 n.2 2011