Data/hora: 02/06/2024 13:06:56

Provedor de dados: | Electronic Journal of Biotechnology
País: | Chile
Título: | Association between AA-NAT gene polymorphism and reproductive performance in sheep
Autores: | Ding-ping,Bai; Cheng-jiang,Yu; Yu-lin,Chen.
Data: | 2012-03-01
Ano: | 2012
Palavras-chave: | AA-NAT gene; Polymorphism; Reproduction; Seasonality; Sheep.
Resumo: | Arylalkylamine N-acetyltransferase (AA-NAT) is critical enzyme in Melatonin (MLT) biosynthesis for MLT regulating the animal seasonal breeding. In this study, DNA sequencing methods were applied to detect the polymorphisms of the AA-NAT gene in 179 Chinese sheep belonging to two non-seasonal reproduction breeds and two seasonal reproduction breeds. One mutation at exon 3 (NM_001009461:c.486A &amp;gt; G) was &amp;#64257;rstly described at the sheep AA-NAT locus. Hence, we described the SmaI PCR-RFLP method for detecting EX3 486A &amp;gt; G mutation, frequencies of the AA-NAT-G allele varied from 0.871 to 0.908 in two non-seasonal reproduction breeds and 0.517 to 0.578 in two seasonal reproduction breeds. The associations of SmaI polymorphism with estrus traits was analyzed in non-seasonal reproduction breeds sheep and seasonal reproduction breeds sheep, the signi&amp;#64257;cant statistical results were found between them, the GG genotype frequencies was higher in non-seasonal reproduction breeds (p &lt; 0.001), while, the GA genotype frequencies was higher in seasonal reproduction breeds (p &lt; 0.05). Hence, the EX3 486A &amp;gt; G mutation could facilitate association analysis and serve as a genetic marker for Chinese sheep breeding and genetics.
Tipo: | Journal article
Idioma: | Inglês
Identificador: | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000200007
Editor: | Pontificia Universidad Católica de Valparaíso
Formato: | text/html
Fonte: | Electronic Journal of Biotechnology  v.15 n.2 2012