Data/hora: 11/06/2024 02:43:09

Provedor de dados: | 69
País: | Chile
Título: | Identification and genetic diversity analysis of Memecylon species using ISSR, RAPD and Gene-Based DNA barcoding tools
Autores: | Tumkur Ramasetty,Bharathi; Naik Bajpe,Shrisha; Kigga Kadappa,Sampath Kumara; Kumar Saini,Ramesh; Nittur Basavaraju,Shashibhushan; Kukkundoor Ramachandra,Kini; Harishchandra Sripathy,Prakash.
Data: | 2016-11-01
Ano: | 2016
Palavras-chave: | AtpF-atpH; Memecylon species; Ndh; PsbA-trnH; RpoC1; 5s; PCR based techniques; Molecular phylogenetic analysis; Molecular phylogenesis; DNA markers.
Resumo: | Background: Memecylon species are commonly used in Indian ethnomedical practices. The accurate identification is vital to enhance the drug's efficacy and biosafety. In the present study, PCR based techniques like RAPD, ISSR and DNA barcoding regions, such as 5s, psbA-trnH, rpoC1, ndh and atpF-atpH, were used to authenticate and analyze the diversity of five Memecylon species collected from Western Ghats of India. Results: Phylogenetic analysis clearly distinguished Memecylon malabaricum from Memecylon wightii and Memecylon umbellatum from Memecylon edule and clades formed are in accordance with morphological keys. In the RAPD and ISSR analyses, 27 accessions representing five Memecylon species were distinctly separated into three different clades. M. malabaricum and M. wightii grouped together and M. umbellatum, M. edule and Memecylon talbotianum grouped in the same clade with high Jaccard dissimilarity coefficient and bootstrap support between each node, indicating that these grouped species are phylogenetically similar. Conclusion: Data from the present study reveals that chloroplast psbA-trnH region could be used as a potential candidate region for identifying Memecylon species, and ISSR marker system could be used for estimating genetic diversity since it has high percent polymorphism compared to RAPD marker.
Tipo: | Journal article
Idioma: | Inglês
Identificador: | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000600001
Editor: | Pontificia Universidad Católica de Valparaíso
Formato: | text/html
Fonte: | Electronic Journal of Biotechnology  v.19 n.6 2016