Data/hora: 04/06/2024 04:30:23

Provedor de dados: 	Electronic Journal of Biotechnology	
País: 	Chile	
Título: 	Isolation of high quality RNA from Polyporus umbellatus (Pers.) Fries	
Autores: 	Zhang,Yuejin; Qin,Yali; Guo,Lijun; Zhou,Zili; Liang,Zongsuo; Zhang,Chenlu; Guo,Hongbo.	
Data: 	2012-09-01	
Ano: 	2012	
Palavras-chave: 	Chuling; Medicinal fungus; Polyporus umbellatus; RNA isolation.	
Resumo: 	Background: The dried sclerotium of medicinal fungus Polyporus umbellatus (Pers.) Fries has many pharmacological functions such as diuretic and anticancer activity, in which high-content polysaccharides may play an important role. However, RNA isolation is difficult in filamentous fungi and lacking in P. umbellatus. Results: Five methods for RNA extraction from five strains collected from four provinces were assessed for their ability to recover a high-quality RNA applicable for sequence-related amplification polymorphism (SRAP) PCR and GDP-D-mannose pyrophosphorylase (GMP) gene expression profiles. Both A260/A280 and A260/A230 ratios of the best Trizol Plus + RNAiso-mate for Plant Tissue method are around 2 with a yield of 1122.00 ± 0.21 ng &amp;#956;l-1. The Trizol method also showed good quality with the yield 469.60 ng &amp;#956;l-1. The SRAP PCR amplified clear and polymorphic bands in all five cDNA samples transcribed from RNA by using primer Me4-Em4. GMP gene fragment (1251 bp) was successfully amplified by RT-PCR, suggesting the integrity of isolated RNA. Conclusion: All these results showed that the total RNA isolated by this protocol is of sufficient quality for subsequent molecular applications.	
Tipo: 	Journal article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000500010	
Editor: 	Pontificia Universidad Católica de Valparaíso	
Formato: 	text/html	
Fonte: 	Electronic Journal of Biotechnology  v.15 n.5 2012