Data/hora: 09/06/2024 17:36:39

Provedor de dados: | 69
País: | Chile
Título: | Biotransformation of 3-hydroxydibenzo-&amp;#945;-pyrone into 3,8 dihydroxydibenzo-&amp;#945;-pyrone and aminoacyl conjugates by Aspergillus niger isolated from native&amp;#8220;shilajit&amp;#8221;
Autores: | Islam,Aminul; Ghosh,Runa; Banerjee,Dipankar; Nath,Piali; Mazumder,Upal Kanti; Ghosal,Shibnath.
Data: | 2008-07-01
Ano: | 2008
Palavras-chave: | Coenzyme Q10 reduction; Electron transport chain humification; Paleohumus.
Resumo: | &amp;#8220;Shilajit&amp;#8221; is a panacea in Ayurveda, the Indian traditional system of medicine. The major bioactives of &amp;#8220;shilajit&amp;#8221; have been identified as dibenzo-&amp;#945;-pyrones (DBPs), its oligomers and aminoacyl conjugated derivatives. These bioactive compounds play a crucial role in energy metabolism in all animal cells including those of man. 3-hydroxydibenzo-&amp;#945;-pyrone (3-OH-DBP), a key DBP component of &amp;#8220;shilajit&amp;#8221; is converted, among other products, to another active DBP derivative, viz. 3,8-hydroxydibenzo-&amp;#945;-pyrone, 3,8(OH)2-DBP, in vivo, when its precursor is ingested. 3,8(OH)2-DBP is then involved in energy synthesis in the mitochondria in the reduction and stabilization of coenzyme Q10 in the electron transport chain. As the chemical synthesis of 3,8(OH)2-DBP is a complex, multi-step process and economically not readily viable, we envisioned the development of a process using microorganisms for bioconversion of 3-OH-DBP to 3,8(OH)2-DBP. In this study, the biotransformation of 3-OH-DBP is achieved using Aspergillus niger, which was involved in the humification process on sedimentary rocks leading to &amp;#8220;shilajit&amp;#8221; formation. A 60% bioconversion of 3-OH-DBP to 3,8(OH)2-DBP and to its aminoacyl derivatives was achieved. The products were characterized and estimated by high performance liquid chromatography (HPLC), high performance flash chromatography (HPFC) and gas chromatography-mass spectrometry (GC-MS) analyses. Among the Aspergillus species isolated and identified from native &amp;#8220;shilajit&amp;#8221;, A. niger was found to be the most efficient for this bioconversion.
Tipo: | Journal article
Idioma: | Inglês
Identificador: | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000300001
Editor: | Pontificia Universidad Católica de Valparaíso
Formato: | text/html
Fonte: | Electronic Journal of Biotechnology  v.11 n.3 2008