Data/hora: 10/06/2024 03:34:36

Provedor de dados: | 69
País: | Chile
Título: | Construction of recombinant Bacillus subtilis strains for efficient pimelic acid synthesis
Autores: | Zhang,Wei-Wei; Yang,Ming-Ming; Li,Heng-xin; Wang,Dun.
Data: | 2011-11-01
Ano: | 2011
Palavras-chave: | Bacillus subtilis; Biosynthesis; Integration; Pglv promoter; Pimelic acid; Single crossover event.
Resumo: | As a precursor, pimelic acid plays an important role in biotin biosynthesis pathway of Bacillus subtilis. Fermentations supplemented with pimelic acid could improve the production of biotin, however, with a disadvantage-high cost. So it is necessary to improve the biosynthesis of pimelic acid via genetic engineering in B. subtilis. In this study, we constructed a recombinant B. subtilis strain for improving the synthesis of pimelic acid, in which a maltose-inducible Pglv promoter was inserted into the upstream of the cistron bioI-orf2-orf3 and, meanwhile, flanked by the tandem cistrons via a single crossover event. The copy number of the integrant was amplified by high-concentration resistance screen and increased to 4-5 copies. The production of pimelic acid from multiple copies integrant was about 4 times higher than that from single copy (1017.13 &amp;#956;g/ml VS. 198.89 &amp;#956;g/ml). And when induced by maltose the production of pimelic acid was about 2 times of that under non-induction conditions (2360.73 &amp;#956;g/ml VS. 991.59 &amp;#956;g/ml). Thus, these results demonstrated that the production of pimelic acid was improved obviously through reconstructed B. subtilis. It also suggested that our expression system provided a convenient source of pimelic acid that would potentially lower the cost of production of biotin from engineered B. subtilis.
Tipo: | Journal article
Idioma: | Inglês
Identificador: | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000600001
Editor: | Pontificia Universidad Católica de Valparaíso
Formato: | text/html
Fonte: | Electronic Journal of Biotechnology  v.14 n.6 2011