Data/hora: 01/06/2024 02:52:04

Provedor de dados: | 69
País: | Chile
Título: | Biochemical properties of an extracellular &amp;#946;-D-fructofuranosidase II produced by Aspergillus phoenicis under Solid-Sate Fermentation using soy bran as substrate
Autores: | Rustiguel,Cynthia Barbosa; Oliveira,Arthur Henrique Cavalcanti de; Terenzi,Héctor Francisco; Jorge,João Atílio; Guimarães,Luis Henrique Souza.
Data: | 2011-03-01
Ano: | 2011
Palavras-chave: | Agroindustrial substrate; Aspergillus; ?-D-fructofuranosidase; Solid state fermentation; Sucrose.
Resumo: | The filamentous fungus A. phoenicis produced high levels of ?-D-fructofuranosidase (FFase) when grown for 72 hrs under Solid-State Fermentation (SSF), using soy bran moistened with tap water (1:0.5 w/v) as substrate/carbon source. Two isoforms (I and II) were obtained, and FFase II was purified 18-fold to apparent homogeneity with 14% recovery. The native molecular mass of the glycoprotein (12% of carbohydrate content) was 158.5 kDa with two subunits of 85 kDa estimated by SDS-PAGE. Optima of temperature and pH were 55Ã¯Â¿Â½C and 4.5. The enzyme was stable for more than 1 hr at 50Ã¯Â¿Â½C and was also stable in a pH range from 7.0 to 8.0. FFase II retained 80% of activity after storage at 4Ã¯Â¿Â½C by 200 hrs. Dichroism analysis showed the presence of random and ?-sheet structure. A. phoenicis FFase II was activated by Mn2+, Mg2+ and Co2+, and inhibited by Cu2+, Hg2+ and EDTA. The enzyme hydrolyzed sucrose, inulin and raffinose. Kd and Vmax values were 18 mM and 189 U/mg protein using sucrose as substrate.
Tipo: | Journal article
Idioma: | Inglês
Identificador: | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000200002
Editor: | Pontificia Universidad Católica de Valparaíso
Formato: | text/html
Fonte: | Electronic Journal of Biotechnology  v.14 n.2 2011