Data/hora: 04/06/2024 16:51:46

Provedor de dados: | 69
País: | Chile
Título: | A new variety of Bacopa monnieri obtained by in vitro polyploidization
Autores: | Salvio Escandón,Alejandro; Hagiwara,Juan Carlos; Alderete,Liliana Marisol.
Data: | 2006-06-01
Ano: | 2006
Palavras-chave: | Colchicine; Flow cytometry; Ornamental plants; Scrophulariaceae; Tetraploid.
Resumo: | The commercial value of Bacopa monnieri, a widespread herbaceous plant in Argentina, can be substantially improved increasing its flower size by chromosome doubling with colchicine. MS supplemented with 0.25 mg/L of 6-benzylaminopurine proved to be an appropriate medium for the in vitro multiplication of nodal segments of B. monnieri. Polyploidization was achieved submerging nodal segments during 24 or 48 hrs in colchicine solution 0.001 and 0.01% P/V, in 1% DMSO. Segments submerged in water and in DMSO 1% aqueous solution were used as controls. DNA contents from recovered plants was measured and characterized and their phenotype described. Two tetraploid plants originated from independent events were detected. These plants showed significant differences in size and colour both in leaves and flowers compared to untreated controls.
Tipo: | Journal article
Idioma: | Inglês
Identificador: | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000300002
Editor: | Pontificia Universidad Católica de Valparaíso
Formato: | text/html
Fonte: | Electronic Journal of Biotechnology  v.9 n.3 2006