Data/hora: 08/06/2024 18:33:53

Provedor de dados: | 69
País: | Chile
Título: | Isolation of simple sequence repeats from groundnut
Autores: | Tang Wang,Chuan; Dao Yang,Xin; Xu Chen,Dian; Lin Yu,Shan; Zhen Liu,Guang; Yi Tang,Yue; Zhi Xu,Jian.
Data: | 2007-07-01
Ano: | 2007
Palavras-chave: | Groundnut; Isolation; Simple sequence repeat.
Resumo: | SSRs have proved to be the most powerful tool for variety identification in groundnut of similar origin, and have much potential in genetic and breeding studies. To facilitate SSR discovery in groundnut, we proposed a highly simplified SSR isolation protocol based on multiple enzyme digestion/ligation, mixed biotin-labeled probes and streptavidin coated magnetic beads hybridization capture strategy. Of the 272 colonies randomly picked for sequencing, 119 were found to have unique SSR inserts
Tipo: | Journal article
Idioma: | Inglês
Identificador: | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000300015
Editor: | Pontificia Universidad Católica de Valparaíso
Formato: | text/html
Fonte: | Electronic Journal of Biotechnology  v.10 n.3 2007