Data/hora: 13/06/2024 01:05:29

Provedor de dados: | Genetics and Molecular Biology
País: | Brazil
Título: | Genetic diversity in natural populations of Jacaranda decurrens Cham. determined using RAPD and AFLP markers
Autores: | Bertoni,Bianca W.; Telles,Mariana P. de C.; Malosso,Milena G.; Torres,Simone C.Z.; Pereira,José O.; Lourenço,Mirian V.; França,Suzelei de C.; Pereira,Ana M.S.
Data: | 2010-01-01
Ano: | 2010
Palavras-chave: | Bignoniaceae; Carobinha; Cerrado; Germplasm bank; Preservation.
Resumo: | Jacaranda decurrens (Bignoniaceae) is an endemic species of the Cerrado with validated antitumoral activity. The genetic diversity of six populations of J. decurrens located in the State of São Paulo was determined in this study by using molecular markers for randomly amplified polymorphic DNA (RAPD) and amplified fragment length polymorphism (AFLP). Following optimization of the amplification reaction, 10 selected primers generated 78 reproducible RAPD fragments that were mostly (69.2%) polymorphic. Two hundred and five reproducible AFLP fragments were generated by using four selected primer combinations; 46.3% of these fragments were polymorphic, indicating a considerable level of genetic diversity. Analysis of molecular variance (AMOVA) using these two groups of markers indicated that variability was strongly structured amongst populations. The unweighted pair group method with arithmatic mean (UPGMA) and Pearson's correlation coefficient (RAPD -0.16, p = 0.2082; AFLP 0.37, p = 0.1006) between genetic matrices and geographic distances suggested that the population structure followed an island model in which a single population of infinite size gave rise to the current populations of J. decurrens, independently of their spatial position. The results of this study indicate that RAPD and AFLP markers were similarly efficient in measuring the genetic variability amongst natural populations of J. decurrens. These data may be useful for developing strategies for the preservation of this medicinal species in the Cerrado.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000300025
Editor: | Sociedade Brasileira de Genética
Relação: | 10.1590/S1415-47572010005000068
Formato: | text/html
Fonte: | Genetics and Molecular Biology  v.33 n.3 2010
Fonte: | info:eu-repo/semantics/openAccess