Data/hora: 08/06/2024 14:04:43

Provedor de dados: 	74	
País: 	Brazil	
Título: 	Downregulation of caffeoyl-CoA O-methyltransferase (CCoAOMT) by RNA interference leads to reduced lignin production in maize straw	
Autores: 	Li,Xiaoyu; Chen,Wenjuan; Zhao,Yang; Xiang,Yan; Jiang,Haiyang; Zhu,Suwen; Cheng,Beijiu.	
Data: 	2013-01-01	
Ano: 	2013	
Palavras-chave: 	CCoAOMT; Lignin biosynthesis; Lignin and cellulose content; Maize.	
Resumo: 	Lignin is a major cell wall component of vascular plants that provides mechanical strength and hydrophobicity to vascular vessels. However, the presence of lignin limits the effective use of crop straw in many agroindustrial processes. Here, we generated transgenic maize plants in which the expression of a lignin biosynthetic gene encoding CCoAOMT , a key enzyme involved in the lignin biosynthesis pathway was downregulated by RNA interference (RNAi). RNAi of CCoAOMT led to significantly downregulated expression of this gene in transgenic maize compared with WT plants. These transgenic plants exhibited a 22.4% decrease in Klason lignin content and a 23.3% increase in cellulose content compared with WT plants, which may reflect compensatory regulation of lignin and cellulose deposition. We also measured the lignin monomer composition of the RNAi plants by GC-MS and determined that transgenic plants had a 57.08% higher S/G ratio than WT plants. In addition, histological staining of lignin with Wiesner reagent produced slightly more coloration in the xylem and sclerenchyma than WT plants. These results provide a foundation for breeding maize with low-lignin content and reveal novel insights about lignin regulation via genetic manipulation of CCoAOMT expression.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000400013	
Editor: 	Sociedade Brasileira de Genética	
Relação: 	10.1590/S1415-47572013005000039	
Formato: 	text/html	
Fonte: 	Genetics and Molecular Biology  v.36 n.4 2013	
Fonte: 	info:eu-repo/semantics/openAccess