Data/hora: 02/06/2024 03:33:12

Provedor de dados: 	Genetics and Molecular Biology	
País: 	Brazil	
Título: 	Sensitivity of RT-PCR method in samples shown to be positive for Zika virus by RT-qPCR in vector competence studies	
Autores: 	Paiva,Marcelo Henrique Santos; Guedes,Duschinka Ribeiro Duarte; Leal,Walter Soares; Ayres,Constância Flávia Junqueira.	
Data: 	2017-09-01	
Ano: 	2017	
Palavras-chave: 	ZIKV; RT-PCR; Aedes aegypti; Vector competence.	
Resumo: 	Abstract Tissue samples from mosquitoes artificially infected with Zika virus and shown to be positive by RT-qPCR were reexamined by RT-PCR. Using these samples we compared the two methods employed in virus RNA detection for vector competence studies. Results demonstrated that, albeit useful, RT-PCR gave false negatives with low viral loads (&lt; 106 RNA copies/ml).	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000400597	
Editor: 	Sociedade Brasileira de Genética	
Relação: 	10.1590/1678-4685-gmb-2016-0312	
Formato: 	text/html	
Fonte: 	Genetics and Molecular Biology  v.40 n.3 2017	
Fonte: 	info:eu-repo/semantics/openAccess