Data/hora: 10/06/2024 19:07:12

Provedor de dados: | Genetics and Molecular Biology
País: | Brazil
Título: | Relationships between chromatin remodeling and DNA damage repair induced by 8-methoxypsoralen and UVA in yeast Saccharomyces cerevisiae
Autores: | Cruz,Lavínia Almeida; Guecheva,Temenouga Nikolova; Bonato,Diego; Henriques,João Antônio Pêgas.
Data: | 2012-01-01
Ano: | 2012
Palavras-chave: | DNA repair; Psoralen; Chromatin remodeling; Histones; DNA interstrand cross-links.
Resumo: | Eukaryotic cells have developed mechanisms to prevent genomic instability, such as DNA damage detection and repair, control of cell cycle progression and cell death induction. The bifunctional compound furocumarin 8-methoxy-psoralen (8-MOP) is widely used in the treatment of various inflammatory skin diseases. In this review, we summarize recent data about the role of chromatin remodeling in the repair of DNA damage induced by treatment with 8-methoxypsoralen plus UVA (8-MOP+UVA), focusing on repair proteins in budding yeast Saccharomyces cerevisiae, an established model system for studying DNA repair pathways. The interstrand crosslinks (ICL) formed by the 8-MOP+UVA treatment are detrimental lesions that can block transcription and replication, leading to cell death if not repaired. Current data show the involvement of different pathways in ICL processing, such as nucleotide excision repair (NER), base excision repair (BER), translesion repair (TLS) and double-strand break repair. 8-MOP+UVA treatment in yeast enhances the expression of genes involved in the DNA damage response, double strand break repair by homologous replication, as well as genes related to cell cycle regulation. Moreover, alterations in the expression of subtelomeric genes and genes related to chromatin remodeling are consistent with structural modifications of chromatin relevant to DNA repair. Taken together, these findings indicate a specific profile in 8-MOP+UVA responses related to chromatin remodeling and DNA repair.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000600021
Editor: | Sociedade Brasileira de Genética
Relação: | 10.1590/S1415-47572012000600021
Formato: | text/html
Fonte: | Genetics and Molecular Biology  v.35 n.4 suppl.1 2012
Fonte: | info:eu-repo/semantics/openAccess