Data/hora: 09/06/2024 04:39:10

Provedor de dados: 	74	
País: 	Brazil	
Título: 	Esterase-3 polymorphism in the sugarcane borer Diatraea saccharalis (Lepidoptera, Pyralidae)	
Autores: 	Ruvolo-Takasusuki,Maria Claudia C.; Machado,Maria de Fátima P.S.; Conte,Hélio.	
Data: 	2002-01-01	
Ano: 	2002	
Palavras-chave: 	Esterase; Genetic variability; Isozymes; Moth; Sugarcane borer.	
Resumo: 	The migration rate of esterases and their substrate specificity for 4-methylumbelliferyl esters (acetate, propionate, and butyrate) and alpha- and beta-naphthyl esters were analyzed in Diatraea saccharalis by starch gel electrophoresis. Substrate preference of esterases was observed with Est-2 and Est-8 isozymes showing substrate specificity for 4-methylumbelliferyl esters and Est-4 isozyme showing specificity for 4-methylumbelliferyl butyrate and alpha-naphthyl butyrate. Allele variation was detected at the Est-3 locus. Two alleles, Est-3F and Est-3S, were identified in pupae with fluorogenic and ester-naphthyl substrates. Chi-square analysis showed no differences between the observed genotypic frequencies and those expected on the basis of Hardy-Weinberg frequencies for the Est-3 locus (chi² = 2.4; p &lt; 0.01). The negative value for the Wright's fixation index (F = -0.2096) calculated for the D. saccharalis population maintained under laboratory conditions indicates an excess of heterozygotes, however, the observed Hardy-Weinberg equilibrium indicates that in the laboratory the population of D. saccharalis behaved as if the moth were randomly mating in nature. The high level of heterozygosity at the Est-3 locus indicates also that this esterase may be a good genetic marker for studies of natural D. saccharalis populations.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000100012	
Editor: 	Sociedade Brasileira de Genética	
Relação: 	10.1590/S1415-47572002000100012	
Formato: 	text/html	
Fonte: 	Genetics and Molecular Biology  v.25 n.1 2002	
Fonte: 	info:eu-repo/semantics/openAccess