Data/hora: 11/06/2024 09:34:53

Provedor de dados: | 74
País: | Brazil
Título: | Cross-amplification and characterization of microsatellite loci for the Neotropical orchid genus Epidendrum
Autores: | Pinheiro,Fábio; Palma-Silva,Clarisse; Barros,Fábio de; Cozzolino,Salvatore.
Data: | 2009-01-01
Ano: | 2009
Palavras-chave: | Epidendrum; Orchidaceae; Short tandem repeat; Cross-amplification.
Resumo: | In this study we tested the cross-amplification of 33 microsatellite loci previously developed for two closely related Neotropical orchid genera (Epidendrum and Laelia). A set of ten loci were polymorphic across five examined species (20 individuals each) with 2 to 15 alleles per locus. The mean expected and observed heterozygosity (average across species) ranged from 0.34 to 0.82 and from 0.27 to 0.85, respectively. In addition we tested all loci in 35 species representative of the genus Epidendrum. Of these, 26 loci showed successful amplification. Cross-application of these loci represent a potential source of co-dominant markers for evolutionary, ecological and conservation studies in this important orchid genus.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000200021
Editor: | Sociedade Brasileira de Genética
Relação: | 10.1590/S1415-47572009005000037
Formato: | text/html
Fonte: | Genetics and Molecular Biology  v.32 n.2 2009
Fonte: | info:eu-repo/semantics/openAccess