Data/hora: 09/06/2024 13:43:35

Provedor de dados: | Genetics and Molecular Biology
País: | Brazil
Título: | Cloning and expression analysis of the chitinase gene Ifu-chit2 from Isaria fumosorosea
Autores: | Meng,Huimin; Wang,Zhangxun; Meng,Xiangyun; Xie,Ling; Huang,Bo.
Data: | 2015-09-01
Ano: | 2015
Palavras-chave: | Isaria fumosorosea; Chitinase; Prokaryotic expression; Quantitative real-time PCR.
Resumo: | Entomopathogenic fungi can produce a series of chitinases, some of which function synergistically with proteases and other hydrolytic enzymes to degrade the insect cuticle. In the present study, the chitinase gene Ifu-chit2 from Isaria fumosorosea was investigated. The Ifu-chit2 gene is 1,435-bp long, interrupted by three short introns, and encodes a predicted protein of 423 amino acids with a 22 residue signal peptide. The predicted Ifu-Chit2 protein is highly homologous to Beauveria bassiana chitinase Bbchit2 and belongs to the glycohydrolase family 18. Ifu-Chit2 was expressed in Escherichia coli to verify chitinase activity, and the recombinant enzyme exhibited activity with a colloidal chitin substrate. Furthermore, the expression profiles of Ifu-chit2 were analyzed at different induction times under in vivo conditions. Quantitative real-time PCR analysis revealed that Ifu-chit2 expression peaked at two days post-induction. The expression of chitinase Ifu-chit2 in vivo suggests that the chitinase may play a role in the early stage of pathogenesis.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000300381
Editor: | Sociedade Brasileira de Genética
Relação: | 10.1590/S1415-475738320150003
Formato: | text/html
Fonte: | Genetics and Molecular Biology  v.38 n.3 2015
Fonte: | info:eu-repo/semantics/openAccess