Data/hora: 10/06/2024 09:13:16

Provedor de dados: 	74	
País: 	Brazil	
Título: 	Cloning and expression analysis of the chitinase gene Ifu-chit2 from Isaria fumosorosea	
Autores: 	Meng,Huimin; Wang,Zhangxun; Meng,Xiangyun; Xie,Ling; Huang,Bo.	
Data: 	2015-09-01	
Ano: 	2015	
Palavras-chave: 	Isaria fumosorosea; Chitinase; Prokaryotic expression; Quantitative real-time PCR.	
Resumo: 	Entomopathogenic fungi can produce a series of chitinases, some of which function synergistically with proteases and other hydrolytic enzymes to degrade the insect cuticle. In the present study, the chitinase gene Ifu-chit2 from Isaria fumosorosea was investigated. The Ifu-chit2 gene is 1,435-bp long, interrupted by three short introns, and encodes a predicted protein of 423 amino acids with a 22 residue signal peptide. The predicted Ifu-Chit2 protein is highly homologous to Beauveria bassiana chitinase Bbchit2 and belongs to the glycohydrolase family 18. Ifu-Chit2 was expressed in Escherichia coli to verify chitinase activity, and the recombinant enzyme exhibited activity with a colloidal chitin substrate. Furthermore, the expression profiles of Ifu-chit2 were analyzed at different induction times under in vivo conditions. Quantitative real-time PCR analysis revealed that Ifu-chit2 expression peaked at two days post-induction. The expression of chitinase Ifu-chit2 in vivo suggests that the chitinase may play a role in the early stage of pathogenesis.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000300381	
Editor: 	Sociedade Brasileira de Genética	
Relação: 	10.1590/S1415-475738320150003	
Formato: 	text/html	
Fonte: 	Genetics and Molecular Biology  v.38 n.3 2015	
Fonte: 	info:eu-repo/semantics/openAccess