Data/hora: 11/06/2024 05:22:29

Provedor de dados: 	Genetics and Molecular Biology	
País: 	Brazil	
Título: 	Specific amplification of iron receptor genes in Xylella fastidiosa strains from different hosts	
Autores: 	Pacheco,Flávia Teresa Hansen; Silva-Stenico,Maria Estela; Etchegaray,Augusto; Gomes,José Elias; Carrilho,Emanuel; Tsai,Siu Mui.	
Data: 	2006-01-01	
Ano: 	2006	
Palavras-chave: 	Citrus variegated chlorosis; Plant pathogen; Iron-transportation; Pyoverdine; Enterobactin; Polyketide synthase; Peptide synthetase.	
Resumo: 	Bacterial production of siderophores may involve specific genes related to nonribosomal peptide and polyketide biosynthesis, which have not been fully identified in the genome of Xylella fastidiosa strain 9a5c. However, a search for siderophore-related genes in strain 9a5c indicated five membrane receptors, including siderophore, ferrichrome-iron and hemin receptors. All these biomolecules are thought to be associated with iron transport and utilization. Eighty isolates obtained from citrus orchards containing trees that developed citrus variegated chlorosis (CVC) were screened for siderophore production. The results demonstrated that only 10 of the isolates did not produce siderophores. Additional strains obtained from coffee, almond, mulberry, elm, ragweed, periwinkle and grape also infected by X. fastidiosa were also shown by the chromeazurol bioassay to produce siderophores. In order to correlate siderophore production with the presence of siderophore-related genes, a polymerase chain reaction (PCR) was developed using specific primers for the catechol-type ferric enterobactin receptor (pfeA) and the hydroxamate-type ferrisiderophore receptor (fiuA) genes of strain 9a5c. The PCR results confirmed our hypothesis by demonstrating that amplification products were detected in all strains except for those isolates that did not produce siderophores.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000100026	
Editor: 	Sociedade Brasileira de Genética	
Relação: 	10.1590/S1415-47572006000100026	
Formato: 	text/html	
Fonte: 	Genetics and Molecular Biology  v.29 n.1 2006	
Fonte: 	info:eu-repo/semantics/openAccess