Data/hora: 02/06/2024 04:46:59

Provedor de dados: | 74
País: | Brazil
Título: | Protocol for extraction of genomic DNA from swine solid tissues
Autores: | Biase,Fernando Henrique; Franco,Maurício Machaim; Goulart,Luiz Ricardo; Antunes,Robson Carlos.
Data: | 2002-01-01
Ano: | 2002
Palavras-chave: | DNA extraction; Swine tissues.
Resumo: | Molecular diagnostics are performed by using DNA from different body tissues. However, it is necessary to obtain genomic DNA of good quality. Due to the impossibility of collecting blood from slaughtered animals, DNA extraction from solid tissues is necessary. The objective of this study was to describe a protocol of DNA extraction from swine skin, adipose, brain, liver, kidney and muscle tissues. We obtained high molecular weight DNA of good quality, shown by agarose gel and amplification of two DNA fragments, 605bp and 891pb, by PCR. Spectrophotometric analysis of DNA concentration showed variation among the DNA from different tissues, with the liver and adipose tissues presenting the greatest and the smallest concentration, respectively. The described protocol has proven to be advantageous due to its simplicity, quickness, affordable reagents and absence of phenol, resulting in a high molecular weight DNA of good quality from several tissues.
Tipo: | Info:eu-repo/semantics/other
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000300011
Editor: | Sociedade Brasileira de Genética
Relação: | 10.1590/S1415-47572002000300011
Formato: | text/html
Fonte: | Genetics and Molecular Biology  v.25 n.3 2002
Fonte: | info:eu-repo/semantics/openAccess