Data/hora: 17/06/2024 02:19:15

Provedor de dados: | 74
País: | Brazil
Título: | Characterization of swine stress gene by DNA testing using plucked hair as a source of DNA
Autores: | Bastos,Reginaldo Gaspar; Federizzi,Joreci; Deschamps,João Carlos; Cardellino,Ricardo; Dellagostin,Odir Antônio.
Data: | 2000-12-01
Ano: | 2000
Resumo: | The swine stress gene (hal) in recessive homozygotes (nn) leads to porcine stress syndrome (PSS), and is associated with pale, soft, exudative pork (PSE). In heterozygosis (Nn) it is linked to poor carcass quality. A total of 179 pigs (86 Large White, 69 Landrace, 12 Duroc and 12 Pietrain) were characterized as normal homozygotes (NN), heterozygotes or recessive homozygotes following amplification of a target region of the hal gene using the polymerase chain reaction (PCR), followed by a restriction endonuclease assay. Plucked hair was used as a source of genomic DNA. The resulting PCR was digested with the restriction enzyme CfoI, followed by agarose gel electrophoresis. Of 179 animals tested, 70% were NN, 28% were Nn, and 2% were nn. The frequency of heterozygotes was higher (P &lt; 0.05) in Landrace (0.43 for Nn) than in Large White pigs (0.09 for Nn). Nine of the 12 Pietrain animals were Nn and three were nn, suggesting a high frequency for the n allele in this breed. These results may be related to the incidence of PSS and PSE in these two breeds, both of which are widely used in breeding programs. The utilization of plucked hair as the source of genomic DNA was a non-invasive and quick method to screen farm animals.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000400018
Editor: | Sociedade Brasileira de Genética
Relação: | 10.1590/S1415-47572000000400018
Formato: | text/html
Fonte: | Genetics and Molecular Biology  v.23 n.4 2000
Fonte: | info:eu-repo/semantics/openAccess