Data/hora: 13/06/2024 23:41:19

Provedor de dados: | 74
País: | Brazil
Título: | Micropropagation of rubber trees (Hevea brasiliensis Muell. Arg.)
Autores: | Mendanha,Asseara Batista Leitão; Torres,Roberto Augusto de Almeida; Freire,Adelson de Barros.
Data: | 1998-09-01
Ano: | 1998
Resumo: | Tissue cultures were established from newly expanded leaves and axillary buds of rubber trees (Hevea brasiliensis Muell. Arg.). Calli formed from these explants, but no regeneration occurred. Shoots were obtained from axillary buds cultured on Murashige and Skoog's (MS) medium (Physiol. Plant. 15: 473-497, 1962) supplemented with 1.0 mg/l kinetin, 1.0 mg/l 2,4-dichlorophenoxyacetic acid (2,4-D), 20 g/l sucrose and 4 g/l Difco agar. Formation of a root similar to a tap root was induced on MS medium supplemented with 5.0 mg/l naphthaleneacetic acid (NAA), 3.0 mg/l indolylbutyric acid (IBA), 50 g/l sucrose and 4 g/l Difco agar. Several types of explants were used in attempts to recover complete rubber tree plants with well-developed tap roots. Leaf explants and axillary buds formed calli on MS basic medium with different combinations of kinetin, benzylaminopurine (BAP), 2,4-D, IBA, NAA and indolylacetic acid (IAA). The antibiotic tetracycline was also used to control possible bacterial infections. However, no antibiotic effect was noted. Calli formation was abundant, but no regeneration was observed when the calli from different media was transferred to MS medium without growth hormones. On this basic medium, callus cultures became necrotic and died. Shoots developed from axillary buds, rooted vigorously when cultured on MS medium with NAA, IAA, and IBA. Based on these results, further studies with commercially important clones should lead to a feasible micropropagation technique.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000300018
Editor: | Sociedade Brasileira de Genética
Relação: | 10.1590/S1415-47571998000300018
Formato: | text/html
Fonte: | Genetics and Molecular Biology  v.21 n.3 1998
Fonte: | info:eu-repo/semantics/openAccess