Data/hora: 08/06/2024 18:39:39

Provedor de dados: | 74
País: | Brazil
Título: | A low-cost apparatus for transforming Drosophila and detecting green fluorescent protein (GFP) genetic markers
Autores: | Deprá,Maríndia; Sepel,Lenira Maria Nunes; Loreto,Élgion Lucio da Silva.
Data: | 2004-01-01
Ano: | 2004
Palavras-chave: | Transgenic Drosophila; PiggyBac; GFP; Transposable elements.
Resumo: | We describe the transformation of white mutant Drosophila simulans with a piggyBac transposon vector and a green fluorescent marker (GFP) and show how to construct inexpensive micro-manipulation and epifluorescence equipment for use in transposon-mediated germ-line transformation. Although the number of G0 adult flies (16) obtained in relation to the number of injected eggs was very low (12.5%) it was comparable to the proportion described by other authors and can be considered as a good rate of transformation.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000100012
Editor: | Sociedade Brasileira de Genética
Relação: | 10.1590/S1415-47572004000100012
Formato: | text/html
Fonte: | Genetics and Molecular Biology  v.27 n.1 2004
Fonte: | info:eu-repo/semantics/openAccess