Data/hora: 09/06/2024 17:07:56

Provedor de dados: | 74
País: | Brazil
Título: | Quantitative real-time PCR for the clinical detection of Helicobacter pylori
Autores: | Ribeiro,Marcelo Lima; Ecclissato,Christina Cunha; Mattos,Ricardo Gabriel; Mendonca,Sergio; Pedrazzoli Jr.,José.
Data: | 2007-03-01
Ano: | 2007
Palavras-chave: | Diagnostic methods; Helicobacter pylori; Real-time PCR.
Resumo: | Accurate diagnosis of Helicobacter pylori infection is very important in both clinical practice and research. We evaluated the sensitivity of real-time PCR (RT-PCR) for the detection and quantification of Helicobacter pylori using DNA from 91 human gastric biopsy samples divided into three groups: 46 biopsies from untreated patients who according to the references methods were considered H. pylori-negative (group A); 35 biopsies from patients previously treated against H. pylori and considered to be cured by "gold standard" tests (group B); and 10 biopsies from patients H. pylori-positive by all available methods (group C). The sensitivity of the RT-PCR assay was higher than that of standard methods. Of the 81 patients considered to be uninfected according to the references methods, 16 were H. pylori-positive by PCR, 10 of which were patients who had received H. pylori eradication therapy and 6 were untreated patients. Based on these findings we recommend that RT-PCR should be use in addition to standard methods in clinical studies to monitor the results of H. pylori eradication therapy.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000300022
Editor: | Sociedade Brasileira de Genética
Relação: | 10.1590/S1415-47572007000300022
Formato: | text/html
Fonte: | Genetics and Molecular Biology  v.30 n.2 2007
Fonte: | info:eu-repo/semantics/openAccess