Data/hora: 02/06/2024 05:13:51

Provedor de dados: 	Genetics and Molecular Biology	
País: 	Brazil	
Título: 	Quantification of bovine cytokine gene expression using real-time RT-PCR methodology	
Autores: 	Zaros,Lilian Giotto; Bricarello,Patrízia Ana; Amarante,Alessandro Francisco Talamini; Coutinho,Luiz Lehmann.	
Data: 	2007-01-01	
Ano: 	2007	
Palavras-chave: 	Cytokine gene expression; Real-time RT-PCR; Cattle; Gastrointestinal nematodes; Bovine cytokines.	
Resumo: 	T cells produce cytokines that affect host response to infection. This paper reports real-time RT-PCR conditions and validation steps for accurate quantification of Bos indicus cytokines, interleukin (IL)-2, IL-4, IL-8, IL12p-35, IL-13, tumoral necrosis factor (TNF)-alpha, interferon (IFN)-gamma, monocyte chemoattractant proteins (MCP)-1 and MCP-2, and the glycoprotein mucin (MUC)-1 in two groups of Nelore cattle, one resistant and the other susceptible to gastrointestinal nematode infections. RPL-19 was shown to be an ideal internal control gene, since its expression was constant across treatments and presented lower variation when compared to the GAPDH gene. The optimized conditions established in the present study can be used to determine the immune response of cattle under different experimental conditions, such as viral, bacterial and parasite infections.	
Tipo: 	Info:eu-repo/semantics/other	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000400012	
Editor: 	Sociedade Brasileira de Genética	
Relação: 	10.1590/S1415-47572007000400012	
Formato: 	text/html	
Fonte: 	Genetics and Molecular Biology  v.30 n.3 2007	
Fonte: 	info:eu-repo/semantics/openAccess