Data/hora: 16/06/2024 17:45:00

Provedor de dados: | 74
País: | Brazil
Título: | The influence of organic solvents on estimates of genotoxicity and antigenotoxicity in the SOS chromotest
Autores: | Quintero,Nathalia; Stashenko,Elena E.; Fuentes,Jorge Luis.
Data: | 2012-01-01
Ano: | 2012
Palavras-chave: | Antigenotoxicity; Genotoxicity; Interference; Mitomycin C; 4-nitro-quinoline-1-oxide; SOS chromotest; Solvents; Vitamin E.
Resumo: | In this work, the toxicity and genotoxicity of organic solvents (acetone, carbon tetrachloride, dichloromethane, dimethylsulfoxide, ethanol, ether and methanol) were studied using the SOS chromotest. The influence of these solvents on the direct genotoxicity induced by the mutagens mitomycin C (MMC) and 4-nitroquinoline-1-oxide (4-NQO) were also investigated. None of the solvents were genotoxic in Escherichia coli PQ37. However, based on the inhibition of protein synthesis assessed by constitutive alkaline phosphatase activity, some solvents (carbon tetrachloride, dimethylsulfoxide, ethanol and ether) were toxic and incompatible with the SOS chromotest. Solvents that were neither toxic nor genotoxic to E. coli (acetone, dichloromethane and methanol) significantly reduced the genotoxicity of MMC and 4-NQO. When these solvents were used to dissolve vitamin E they increased the antigenotoxic activity of this compound, possibly through additive or synergistic effects. The relevance of these results is discussed in relation to antigenotoxic studies. These data indicate the need for careful selection of an appropriate diluent for the SOS chromotest since some solvents can modulate genotoxicity and antigenotoxicity.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000300018
Editor: | Sociedade Brasileira de Genética
Relação: | 10.1590/S1415-47572012000300018
Formato: | text/html
Fonte: | Genetics and Molecular Biology  v.35 n.2 2012
Fonte: | info:eu-repo/semantics/openAccess