Data/hora: 13/06/2024 01:10:31

Provedor de dados: 	Genetics and Molecular Biology	
País: 	Brazil	
Título: 	Molecular characterization of beta-tubulin from Phakopsora pachyrhizi, the causal agent of Asian soybean rust	
Autores: 	Maciel,Talles Eduardo Ferreira; Freire,Maíra Cristina Menezes; Almeida,Álvaro M.R. de; Oliveira,Luiz Orlando de.	
Data: 	2010-01-01	
Ano: 	2010	
Palavras-chave: 	Asian soybean rust; Beta-tubulin; Genetic diversity; Phakopsora pachyrhizi; Soybeans.	
Resumo: 	β-tubulins are structural components of microtubules and the targets of benzimidazole fungicides used to control many diseases of agricultural importance. Intron polymorphisms in the intron-rich genes of these proteins have been used in phylogeographic investigations of phytopathogenic fungi. In this work, we sequenced 2764 nucleotides of the β-tubulin gene (Pp tubB) in samples of Phakopsora pachyrhizi collected from seven soybean fields in Brazil. Pp tubB contained an open reading frame of 1341 nucleotides, including nine exons and eight introns. Exon length varied from 14 to 880 nucleotides, whereas intron length varied from 76 to 102 nucleotides. The presence of only four polymorphic sites limited the usefulness of Pp tubB for phylogeographic studies in P. pachyrhizi. The gene structures of Pp tubB and orthologous β-tubulin genes of Melampsora lini and Uromyces viciae-fabae were highly conserved. The amino acid substitutions in β-tubulin proteins associated with the onset of benzimidazole resistance in model organisms, especially at His6, Glu198 and Phe200, were absent from the predicted sequence of the P. pachyrhizi β-tubulin protein.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000200025	
Editor: 	Sociedade Brasileira de Genética	
Relação: 	10.1590/S1415-47572010005000040	
Formato: 	text/html	
Fonte: 	Genetics and Molecular Biology  v.33 n.2 2010	
Fonte: 	info:eu-repo/semantics/openAccess