Data/hora: 04/06/2024 04:00:42

Provedor de dados: | 74
País: | Brazil
Título: | The gene expression profiles of induced pluripotent stem cells (iPSCs) generated by a non-integrating method are more similar to embryonic stem cells than those of iPSCs generated by an integrating method
Autores: | Liu,Yajun; Cheng,De; Li,Zhenzhen; Gao,Xing; Wang,Huayan.
Data: | 2012-01-01
Ano: | 2012
Palavras-chave: | DNA microarray analysis; Embryonic stem cells; Gene expression profiling; Induced pluripotent stem cells.
Resumo: | Induced pluripotent stem cells (iPSCs) obtained by the ectopic expression of defined transcription factors have tremendous promise and therapeutic potential for regenerative medicine. Many studies have highlighted important differences between iPSCs and embryonic stem cells (ESCs). In this work, we used meta-analysis to compare the global transcriptional profiles of human iPSCs from various cellular origins and induced by different methods. The induction strategy affected the quality of iPSCs in terms of transcriptional signatures. The iPSCs generated by non-integrating methods were closer to ESCs in terms of transcriptional distance than iPSCs generated by integrating methods. Several pathways that could be potentially useful for studying the molecular mechanisms underlying transcription factor-mediated reprogramming leading to pluripotency were also identified. These pathways were mostly associated with the maintenance of ESC pluripotency and cancer regulation. Numerous genes that are up-regulated during the induction of reprogramming also have an important role in the success of human preimplantation embryonic development. Our results indicate that hiPSCs maintain their pluripotency through mechanisms similar to those of hESCs.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000400022
Editor: | Sociedade Brasileira de Genética
Relação: | 10.1590/S1415-47572012005000050
Formato: | text/html
Fonte: | Genetics and Molecular Biology  v.35 n.3 2012
Fonte: | info:eu-repo/semantics/openAccess