Data/hora: 10/06/2024 06:46:23

Provedor de dados: 	Genetics and Molecular Biology	
País: 	Brazil	
Título: 	Production of recombinant proteins in Escherichia coli	
Autores: 	Schumann,Wolfgang; Ferreira,Luis Carlos S.	
Data: 	2004-01-01	
Ano: 	2004	
Palavras-chave: 	Expression vectors; Secretion; Molecular chaperones.	
Resumo: 	Attempts to obtain a recombinant protein using prokaryotic expression systems can go from a rewarding and rather fast procedure to a frustrating time-consuming experience. In most cases production of heterologous proteins in Escherichia coli K12 strains has remained an empirical exercise in which different systems are tested without a careful insight into the various factors affecting adequate expression of the encoded protein. The present review will deal with E. coli as protein factory and will cover some of the aspects related to transcriptional and translational expression signals, factors affecting protein stability and solubility and targeting of proteins to different cell compartments. Based on the knowledge accumulated over the last decade, we believe that the rate of success for those dedicated to expression of recombinant proteins based on the use E. coli strains can still be significantly improved.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000300022	
Editor: 	Sociedade Brasileira de Genética	
Relação: 	10.1590/S1415-47572004000300022	
Formato: 	text/html	
Fonte: 	Genetics and Molecular Biology  v.27 n.3 2004	
Fonte: 	info:eu-repo/semantics/openAccess