Data/hora: 10/06/2024 01:29:43

Provedor de dados: | 74
País: | Brazil
Título: | Chromosomal localization and molecular marker development of the lipopolysaccharide and beta-1,3-glucan binding protein gene in the Zhikong scallop Chlamys farreri (Jones et Preston) (Pectinoida, Pectinidae)
Autores: | Huan,Pin; Zhang,Xiaojun; Li,Fuhua; Zhang,Yang; Zhao,Cui; Xiang,Jianhai.
Data: | 2010-01-01
Ano: | 2010
Palavras-chave: | Chlamys farreri; LGBP; Fluorescence in situ hybridization (FISH); Indel; SNP.
Resumo: | Zhikong scallop Chlamys farreri (Jones et Preston) is an economically important species in China. Understanding its immune system would be of great help in controlling diseases. In the present study, an important immunity-related gene, the Lipopolysaccharide and Beta-1,3-glucan Binding Protein (LGBP) gene, was located on C. farreri chromosomes by mapping several lgbp-containing BAC clones through fluorescence in situ hybridization (FISH). Through the localization of various BAC clones, it was shown that only one locus of this gene existed in the genome of C. farreri, and that this was located on the long arm of a pair of homologous chromosomes. Molecular markers, consisting of eight single nucleotide polymorphism (SNPs) markers and one insertion-deletion (indel), were developed from the LGBP gene. Indel marker testing in an F1 family revealed slightly distorted segregation (p = 0.0472). These markers can be used to map the LGBP gene to the linkage map and assign the linkage group to the corresponding chromosome. Segregation distortion of the indel marker indicated genes with deleterious alleles might exist in the surrounding region of the LGBP gene.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000100008
Editor: | Sociedade Brasileira de Genética
Relação: | 10.1590/S1415-47572010005000015
Formato: | text/html
Fonte: | Genetics and Molecular Biology  v.33 n.1 2010
Fonte: | info:eu-repo/semantics/openAccess