Data/hora: 10/06/2024 11:23:50

Provedor de dados: | Genetics and Molecular Biology
País: | Brazil
Título: | Expression profile of genes associated with mastitis in dairy cattle
Autores: | Fonseca,Isabela; Silva,Priscila Vendramini; Lange,Carla Christine; Guimarães,Marta F.M.; Weller,Mayara Morena Del Cambre Amaral; Sousa,Katiene Régia Silva; Lopes,Paulo Sávio; Guimarães,José Domingos; Guimarães,Simone E.F.
Data: | 2009-01-01
Ano: | 2009
Palavras-chave: | Cytokine; Gene expression; Immune response; Real-time PCR.
Resumo: | In order to characterize the expression of genes associated with immune response mechanisms to mastitis, we quantified the relative expression of the IL-2, IL-4, IL-6, IL-8, IL-10, IFN-γ and TNF-α genes in milk cells of healthy cows and cows with clinical mastitis. Total RNA was extracted from milk cells of six Black and White Holstein (BW) cows and six Gyr cows, including three animals with and three without mastitis per breed. Gene expression was analyzed by real-time PCR. IL-10 gene expression was higher in the group of BW and Gyr cows with mastitis compared to animals free of infection from both breeds (p &lt; 0.05). It was also higher in BW Holstein animals with clinical mastitis (p &lt; 0.001), but it was not significant when Gyr cows with and without mastitis were compared (0.05 &lt; p &lt; 0.10). Among healthy cows, BW Holstein animals tended to present a higher expression of all genes studied, with a significant difference for the IL-2 and IFN-γ genes (p &lt; 0.001). For animals with mastitis no significant difference in gene expression was observed between the two breeds. These findings suggest that animals with mastitis develop a preferentially cell-mediated immune response. Further studies including larger samples are necessary to better characterize the gene expression profile in cows with mastitis.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000400018
Editor: | Sociedade Brasileira de Genética
Relação: | 10.1590/S1415-47572009005000074
Formato: | text/html
Fonte: | Genetics and Molecular Biology  v.32 n.4 2009
Fonte: | info:eu-repo/semantics/openAccess