Data/hora: 08/06/2024 03:07:52

Provedor de dados: | Genetics and Molecular Biology
País: | Brazil
Título: | YUCCA4 overexpression modulates auxin biosynthesis and transport and influences plant growth and development via crosstalk with abscisic acid in Arabidopsis thaliana
Autores: | Munguía-Rodríguez,Aarón Giovanni; López-Bucio,Jesús Salvador; Ruiz-Herrera,León Francisco; Ortiz-Castro,Randy; Guevara-García,Ángel Arturo; Marsch-Martínez,Nayelli; Carreón-Abud,Yazmín; López-Bucio,José; Martínez-Trujillo,Miguel.
Data: | 2020-01-01
Ano: | 2020
Palavras-chave: | Arabidopsis; Auxin; Abscisic acid; YUCCA4; Root growth; Germination.
Resumo: | Abstract Auxin regulates a plethora of events during plant growth and development, acting in concert with other phytohormones. YUCCA genes encode flavin monooxygenases that function in tryptophan-dependent auxin biosynthesis. To understand the contribution of the YUCCA4 (YUC4) gene on auxin homeostasis, plant growth and interaction with abscisic acid (ABA) signaling, 35S::YUC4 seedlings were generated, which showed elongated hypocotyls with hyponastic leaves and changes in root system architecture that correlate with enhanced auxin responsive gene expression. Differential expression of PIN1, 2, 3 and 7 auxin transporters was detected in roots of YUC4 overexpressing seedlings compared to the wild-type: PIN1 was down-regulated whereas PIN2, PIN3 and PIN7 were up-regulated. Noteworthy, 35S::YUC4 lines showed enhanced sensitivity to ABA on seed germination and post-embryonic root growth, involving ABI4 transcription factor. The auxin reporter genes DR5::GUS, DR5::GFP and BA3::GUS further revealed that abscisic acid impairs auxin responses in 35S::YUC4 seedlings. Our results indicate that YUC4 overexpression influences several aspects of auxin homeostasis and reveal the critical roles of ABI4 during auxin-ABA interaction in germination and primary root growth.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000100302
Editor: | Sociedade Brasileira de Genética
Relação: | 10.1590/1678-4685-gmb-2019-0221
Formato: | text/html
Fonte: | Genetics and Molecular Biology  v.43 n.1 2020
Fonte: | info:eu-repo/semantics/openAccess