Data/hora: 29/05/2024 05:31:21

Provedor de dados: 	Genetics and Molecular Biology	
País: 	Brazil	
Título: 	Classical and molecular cytogenetic analysis in head and neck squamous cell carcinomas	
Autores: 	Veiga,Luciana CS; Bérgamo,Nádia A; Kowalski,Luiz Paulo; Rogatto,Silvia R.	
Data: 	2003-01-01	
Ano: 	2003	
Palavras-chave: 	FISH; Chromosomal aberrations; Head and neck cancer; Chromosome 17.	
Resumo: 	Head and neck carcinomas represent the sixth most frequent type of cancer in the world, and 90% are derived from squamous cells (HNSCC). In this study of 15 HNSCC cases, extensive aneuploidy was detected by G banding in most tumors. The most frequently observed numerical changes involved gain of a chromosome 22, and loss of chromosomes Y, 10, 17, and 19. The most frequent structural alteration was del(22)(q13.1). As compared to G-banding, fluorescence in situ hybridization (FISH) proved to be an effective technique for detecting aneuploidy. Interphase FISH with a chromosome 17 centromere probe disclosed a high frequency of monosomy for chromosome 17, in contrast with G-banding, by which clonal monosomy 17 was detected in only three of the tumors. Painting probes for chromosomes 5 and 16 were used to evaluate a selected series of HNSCC in which G-banding analysis had shown marker chromosomes. FISH analysis failed to confirm the origin of the marker chromosomes, but four out of five cases showed a significant loss of chromosomes 5. This difference between FISH and G-banding results may reflect the smaller number of metaphase analyzed as well as the criteria adopted for sorting these metaphases. Therefore results obtained solely by G-banding analysis should be considered with caution. Our data confirmed the involvement of chromosome 17 in head and neck squamous cell carcinomas.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000200003	
Editor: 	Sociedade Brasileira de Genética	
Relação: 	10.1590/S1415-47572003000200003	
Formato: 	text/html	
Fonte: 	Genetics and Molecular Biology  v.26 n.2 2003	
Fonte: 	info:eu-repo/semantics/openAccess