Data/hora: 11/06/2024 04:04:49

Provedor de dados: 	74	
País: 	Brazil	
Título: 	A study of the constitutive heterochromatin and nucleolus organizer regions of Isocopris inhiata and Diabroctis mimas (Coleoptera: Scarabaeidae, Scarabaeinae) using C-banding, AgNO3 staining and FISH techniques	
Autores: 	Bione,Edgar; Camparoto,Marjori Leiva; Simões,Zilá Luz Paulino.	
Data: 	2005-03-01	
Ano: 	2005	
Palavras-chave: 	Coleoptera; Heterochromatin; NORs; FISH.	
Resumo: 	Meiotic and mitotic chromosomes of Isocopris inhiata and Diabroctis mimas were studied by standard staining procedures, C-banding, silver nitrate staining and FISH using Apis mellifera 28S rDNA as probe. Isocopris inhiata presented a 2n = 18 (8II+ Xy p) karyotype, composed of meta-submetacentric chromosomes with gradual reduction in size. The karyotype of D. mimas was 2n = 20 (9II+ Xy p), composed of meta-submetacentric (pairs 1, 2, 3, 4 and 7) and acrocentric (pairs 5, 6, 8 and 9) chromosomes, with gradual reduction in size. Analysis of constitutive heterochromatin revealed similar C-banding patterns for both species, showing pericentromeric and telomeric bands and diphasic chromosomes. In addition, the X chromosomes of these species were found to be almost completely heterochromatic. The presence of chromocenters was checked in one or more phases of prophase I of these species. All heterochromatin reacted positively for the silver stain. By FISH analysis we were able to locate the rDNA in medium-size autosome pairs in both species and in the X chromosome of D. mimas.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000100019	
Editor: 	Sociedade Brasileira de Genética	
Relação: 	10.1590/S1415-47572005000100019	
Formato: 	text/html	
Fonte: 	Genetics and Molecular Biology  v.28 n.1 2005	
Fonte: 	info:eu-repo/semantics/openAccess