Data/hora: 02/06/2024 10:44:05

Provedor de dados: | 74
País: | Brazil
Título: | Co-transformation of a tropical maize endophytic isolate of Fusarium verticillioides (synonym F. moniliforme) with gusA and nia genes
Autores: | Pamphile,João A.; Rocha,Carmen Lúcia M.S.C. da; Azevedo,João L.
Data: | 2004-01-01
Ano: | 2004
Palavras-chave: | Co-transformation; Gus; Nia; Fusarium verticillioides; Endophyte.
Resumo: | A tropical endophytic isolate of the fungus Fusarium verticillioides (synonym Fusarium moniliforme) obtained from Zea mays was co-transformed with plasmid pNH24 containing the Fusarium oxysporum nitrate reductase nia gene and plasmid pNOM 102 carrying the Escherichia coli b-glucuronidase gusA gene. Transformation frequency for the nia marker was 75 transformants mg-1 vector DNA and introduction of the gusA gene by co-transformation was 57.2% as indicated by the presence of the GUS+ phenotype on plates. Southern analyses confirmed the integration of both plasmids into the genome of ten GUS+ transformants. All co-transformants showed mitotic stability in respect of the GUS+ phenotype. To assess the potential of transformed endophytic fungi as vectors for introducing desirable characteristics into host tropical plants of biotechnological and agricultural importance we successfully infected maize roots and detected GUS+ phenotype”.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000200021
Editor: | Sociedade Brasileira de Genética
Relação: | 10.1590/S1415-47572004000200021
Formato: | text/html
Fonte: | Genetics and Molecular Biology  v.27 n.2 2004
Fonte: | info:eu-repo/semantics/openAccess