Data/hora: 10/06/2024 17:06:01

Provedor de dados: 	Genetics and Molecular Biology	
País: 	Brazil	
Título: 	Co-transformation of a tropical maize endophytic isolate of Fusarium verticillioides (synonym F. moniliforme) with gusA and nia genes	
Autores: 	Pamphile,João A.; Rocha,Carmen Lúcia M.S.C. da; Azevedo,João L.	
Data: 	2004-01-01	
Ano: 	2004	
Palavras-chave: 	Co-transformation; Gus; Nia; Fusarium verticillioides; Endophyte.	
Resumo: 	A tropical endophytic isolate of the fungus Fusarium verticillioides (synonym Fusarium moniliforme) obtained from Zea mays was co-transformed with plasmid pNH24 containing the Fusarium oxysporum nitrate reductase nia gene and plasmid pNOM 102 carrying the Escherichia coli b-glucuronidase gusA gene. Transformation frequency for the nia marker was 75 transformants mg-1 vector DNA and introduction of the gusA gene by co-transformation was 57.2% as indicated by the presence of the GUS+ phenotype on plates. Southern analyses confirmed the integration of both plasmids into the genome of ten GUS+ transformants. All co-transformants showed mitotic stability in respect of the GUS+ phenotype. To assess the potential of transformed endophytic fungi as vectors for introducing desirable characteristics into host tropical plants of biotechnological and agricultural importance we successfully infected maize roots and detected GUS+ phenotype”.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000200021	
Editor: 	Sociedade Brasileira de Genética	
Relação: 	10.1590/S1415-47572004000200021	
Formato: 	text/html	
Fonte: 	Genetics and Molecular Biology  v.27 n.2 2004	
Fonte: 	info:eu-repo/semantics/openAccess