Data/hora: 10/06/2024 06:42:51

Provedor de dados: | Genetics and Molecular Biology
País: | Brazil
Título: | Use of chromosome microdissection in fish molecular cytogenetics
Autores: | Henning,Frederico; Trifonov,Vladimir; Almeida-Toledo,Lurdes Foresti de.
Data: | 2008-01-01
Ano: | 2008
Palavras-chave: | Fluorescence in situ hybridization; Chromosome painting; Sex chromosomes; Cross-FISH.
Resumo: | Chromosome microdissection is a technique in which whole chromosomes or chromosomal segments are dissected under an inverted microscope yielding chromosome-specific sequences. Several protocol modifications introduced during the past 15 years reduced the number of chromosomes required for most applications. This is of particular interest to fish molecular cytogenetics, since most species present highly uniform karyotypes which make impossible the collection of multiple copies of the same chromosome. Probes developed in this manner can be used to investigate chromosome homologies in closely related species. Here we describe a protocol recently used in the gymnotiform species group Eigenmannia and review the major steps involved in the generation of these markers focusing on protocol modifications aiming to reduce the number of required chromosomes.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000200022
Editor: | Sociedade Brasileira de Genética
Relação: | 10.1590/S1415-47572008000200022
Formato: | text/html
Fonte: | Genetics and Molecular Biology  v.31 n.1 suppl.0 2008
Fonte: | info:eu-repo/semantics/openAccess