Data/hora: 09/06/2024 15:36:54

Provedor de dados: | Genetics and Molecular Biology
País: | Brazil
Título: | Eucalyptus ESTs corresponding to the enzyme glutamine synthetase and the protein D1, sites of action of herbicides that cause oxidative stress
Autores: | Velini,Edivaldo Domingues; Trindade,Maria Lúcia Bueno; Alves,Elza; Catâneo,Ana Catarina; Marino,Celso Luis; Maia,Ivan de Godoy; Mori,Edson Seizo; Furtado,Edson Luiz; Guerrini,Iraê Amaral; Wilcken,Carlos Frederico.
Data: | 2005-01-01
Ano: | 2005
Palavras-chave: | Eucalyptus; Glutamine synthetase; D1 protein; Herbicide.
Resumo: | This work was aimed at locating Eucalyptus ESTs corresponding to the GS enzyme (Glutamine Synthetase, EC = 6.3.1.2) and to the D1 protein, which are directly related to resistance to herbicides that promote oxidative stress. Glutamine Synthetase corresponds to the site of action of the herbicide glufosinate. Herbicides that belong to groups such as ureas, uracils, triazines and triazinones act on the D1-Qb complex (receptor of electrons from the Photosystem II) by inactivating it. The clusters EGEQRT3302E01.g, EGEQRT3001F12.b; EGEZLV1203B04.g; EGBGFB1211H06.g and EGEZLV1205F09.g enclosed complete sequences (with 356 amino acids) of the Glutamine Synthetase enzyme. The cluster EGEQSL1054G06.g is a consensus of four reads and enclosed a complete sequence of D1 Protein (with 353 amino acids). The comparison of the sequences of Protein D1 from different species showed that the substitutions of serine (S) by glycine (G) or serine (S) by threonine (T) at the position 264 could produce plants resistant to herbicides that act on electron flow on Photosystem II. The sequence of amino acids corresponding to the cluster EGEQSL1054G06.g had a serine in position 264 indicating sensitivity of the Eucalyptus plants to herbicides that act on this site.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000400010
Editor: | Sociedade Brasileira de Genética
Relação: | 10.1590/S1415-47572005000400010
Formato: | text/html
Fonte: | Genetics and Molecular Biology  v.28 n.3 suppl.0 2005
Fonte: | info:eu-repo/semantics/openAccess