Data/hora: 11/06/2024 04:11:12

Provedor de dados: | Genetics and Molecular Biology
País: | Brazil
Título: | DNA methylation modulates H19 and IGF2 expression in porcine female eye
Autores: | Wang,Dongxu; Wang,Guodong; Yang,Hao; Liu,Haibo; Li,Cuie; Li,Xiaolan; Lin,Chao; Song,Yuning; Li,Zhanjun; Liu,Dianfeng.
Data: | 2017-03-01
Ano: | 2017
Palavras-chave: | H19/IGF2; Gene expression; DNA methylation; Parthenogenetic; Pig.
Resumo: | Abstract The sexually dimorphic expression of H19/IGF2 is evolutionarily conserved. To investigate whether the expression of H19/IGF2 in the female porcine eye is sex-dependent, gene expression and methylation status were evaluated using quantitative real-time PCR (qPCR) and bisulfite sequencing PCR (BSP). We hypothesized that H19/IGF2 might exhibit a different DNA methylation status in the female eye. In order to evaluate our hypothesis, parthenogenetic (PA) cells were used for analysis by qPCR and BSP. Our results showed that H19 and IGF2 were over-expressed in the female eye compared with the male eye (3-fold and 2-fold, respectively). We observed a normal monoallelic methylation pattern for H19 differentially methylated regions (DMRs). Compared with H19 DMRs, IGF2 DMRs showed a different methylation pattern in the eye. Taken together, these results suggest that elevated expression of H19/IGF2 is caused by a specific chromatin structure that is regulated by the DNA methylation status of IGF2 DMRs in the female eye.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000100153
Editor: | Sociedade Brasileira de Genética
Relação: | 10.1590/1678-4685-gmb-2016-0194
Formato: | text/html
Fonte: | Genetics and Molecular Biology  v.40 n.1 2017
Fonte: | info:eu-repo/semantics/openAccess