Data/hora: 10/06/2024 13:48:31

Provedor de dados: | Genetics and Molecular Biology
País: | Brazil
Título: | Peripheral position of CCND1 and HER-2/neu oncogenes within chromosome territories in esophageal and gastric cancers non-related to amplification and overexpression
Autores: | Bizari,Lucimari; Tajara,Eloiza Helena; Silva,Ana Elizabete.
Data: | 2009-01-01
Ano: | 2009
Palavras-chave: | CCND1; HER-2/neu; Chromosome territories; Esophageal carcinoma; Gastric cancer.
Resumo: | Interphase chromosomes have been shown to occupy discrete regions of the nucleus denominated chromosome territories (CTs), their active genes being preferentially positioned on the surfaces of these CTs, where they are accessible to transcriptional machinery. By means of FISH (Fluorescence in situ Hybridization), we analyzed the CCND1 and HER-2/neu gene positions within the CTs and their relationship with gene amplification and protein over-expression in esophageal and gastric cancers. The CCND1 and HER-2/Neu genes were more often positioned at the periphery (mean frequency of 60%-83%) of the CTs in tumor tissues of the esophagus and stomach. Moreover, this positioning revealed no association with either gene amplification or the protein over-expression status of these genes, although, in esophageal carcinoma, Kappa statistics showed a moderate agreement between amplification of the CCND1 gene (Kappa = 0.400) and its location within the CT, as well as with over-expression of the corresponding protein (Kappa = 0.444). Thus, our results suggest that gene positioning in interphase chromosomes does not follow a definitive pattern neither does it depend only on gene transcriptional activity. Apparently, this positioning could be both gene- and tissue-specific, and depends on other factors acting together, such as dense-gene, chromosome size, chromatin structure, and the level and stability of its expression.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000200006
Editor: | Sociedade Brasileira de Genética
Relação: | 10.1590/S1415-47572009005000034
Formato: | text/html
Fonte: | Genetics and Molecular Biology  v.32 n.2 2009
Fonte: | info:eu-repo/semantics/openAccess