Data/hora: 02/06/2024 06:47:36

Provedor de dados: 	74	
País: 	Brazil	
Título: 	In situ DNA transfer to chicken embryos by biolistics	
Autores: 	Ribeiro,Luciana A.; Azevedo,João L.; Aragão,Francisco J.L.; Rech,Elibio L.; Schmidt,Gilberto S.; Coutinho,Luiz L.	
Data: 	1999-12-01	
Ano: 	1999	
Resumo: 	Fertilized chicken eggs were bombarded with a biolistic device. Transient expression of the lacZ gene under the control of a human cytomegalovirus (CMV) promoter was assessed after in situ gene transfer using this approach. The influence of different pressures, vacuum levels and particles was tested. Survival rate improved as particle velocity decreased, but resulted in lower levels of expression. The best survival and expression were obtained with gold particles, a helium gas pressure of 600 psi and a vacuum of 600 mmHg. Under these conditions, all bombarded embryos showed &lt;FONT FACE="Symbol"&gt;b&lt;/FONT&gt;-galactosidase activity, indicating that this was an effective method for transformation of chicken embryos.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000400010	
Editor: 	Sociedade Brasileira de Genética	
Relação: 	10.1590/S1415-47571999000400010	
Formato: 	text/html	
Fonte: 	Genetics and Molecular Biology  v.22 n.4 1999	
Fonte: 	info:eu-repo/semantics/openAccess