Data/hora: 16/06/2024 00:45:07

Provedor de dados: | 74
País: | Brazil
Título: | Chromosomal damage and apoptosis analysis in exfoliated oral epithelial cells from mouthwash and alcohol users
Autores: | Rocha,Rodrigo dos Santos; Meireles,José Roberto Cardoso; Cerqueira,Eneida de Moraes Marcílio.
Data: | 2014-12-01
Ano: | 2014
Palavras-chave: | Micronucleus; Apoptosis; Mouthwash; Alcoholic beverages.
Resumo: | Chromosomal damage and apoptosis were analyzed in users of mouthwash and/or alcoholic beverages, using the micronucleus test on exfoliated oral mucosa cells. Samples from four groups of 20 individuals each were analyzed: three exposed groups (EG1, EG2 and EG3) and a control group (CG). EG1 comprised mouthwash users; EG2 comprised drinkers, and EG3 users of both mouthwashes and alcoholic beverages. Cell material was collected by gently scraping the insides of the cheeks. Then the cells were fixed in a methanol/acetic acid (3:1) solution and stained and counterstained, respectively, with Schiff reactive and fast green. Endpoints were computed on 2,000 cells in a blind test. Statistical analysis showed that chromosomal damage and apoptosis were significantly higher in individuals of groups EG1 and EG3 than in controls (p &lt; 0.005 and p &lt; 0.001, respectively). No significant difference in chromosomal damage and apoptosis was observed between the exposed groups. In EG2, only the occurrence of apoptosis was significantly higher than in the controls. These results suggest that mouthwashes alone or in association with alcoholic drinks induce genotoxic effects, manifested as chromosomal damage and apoptosis. They also suggest that alcoholic drinks are effective for stimulating the process of apoptosis. However, these data need to be confirmed in larger samples.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000500013
Editor: | Sociedade Brasileira de Genética
Relação: | 10.1590/S1415-47572014005000022
Formato: | text/html
Fonte: | Genetics and Molecular Biology  v.37 n.4 2014
Fonte: | info:eu-repo/semantics/openAccess