Data/hora: 10/06/2024 15:03:19

Provedor de dados: | Genetics and Molecular Biology
País: | Brazil
Título: | Sequence characterization, in silico mapping and cytosine methylation analysis of markers linked to apospory in Paspalum notatum
Autores: | Podio,Maricel; Rodríguez,María P.; Felitti,Silvina; Stein,Juliana; Martínez,Eric J.; Siena,Lorena A.; Quarin,Camilo L.; Pessino,Silvina C.; Ortiz,Juan Pablo A.
Data: | 2012-01-01
Ano: | 2012
Palavras-chave: | Apomixis; Chromosome walking; Gene mapping; Molecular markers.
Resumo: | In previous studies we reported the identification of several AFLP, RAPD and RFLP molecular markers linked to apospory in Paspalum notatum. The objective of this work was to sequence these markers, obtain their flanking regions by chromosome walking and perform an in silico mapping analysis in rice and maize. The methylation status of two apospory-related sequences was also assessed using methylation-sensitive RFLP experiments. Fourteen molecular markers were analyzed and several protein-coding sequences were identified. Copy number estimates and RFLP linkage analysis showed that the sequence PnMAI3 displayed 2-4 copies per genome and linkage to apospory. Extension of this marker by chromosome walking revealed an additional protein-coding sequence mapping in silico in the apospory-syntenic regions of rice and maize. Approximately 5 kb corresponding to different markers were characterized through the global sequencing procedure. A more refined analysis based on sequence information indicated synteny with segments of chromosomes 2 and 12 of rice and chromosomes 3 and 5 of maize. Two loci associated with apomixis locus were tested in methylation-sensitive RFLP experiments using genomic DNA extracted from leaves. Although both target sequences were methylated no methylation polymorphisms associated with the mode of reproduction were detected.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000500018
Editor: | Sociedade Brasileira de Genética
Relação: | 10.1590/S1415-47572012005000070
Formato: | text/html
Fonte: | Genetics and Molecular Biology  v.35 n.4 2012
Fonte: | info:eu-repo/semantics/openAccess