Data/hora: 05/06/2024 19:17:30

Provedor de dados: | 74
País: | Brazil
Título: | DNA damage in the kidney tissue cells of the fish Rhamdia quelen after trophic contamination with aluminum sulfate
Autores: | Klingelfus,Tatiane; Costa,Paula Moiana da; Scherer,Marcos; Cestari,Marta Margarete.
Data: | 2015-12-01
Ano: | 2015
Palavras-chave: | Fish; Metal contamination; Chromosomal abnormalities; Comet assay.
Resumo: | Abstract Even though aluminum is the third most common element present in the earth's crust, information regarding its toxicity remains scarce. It is known that in certain cases, aluminum is neurotoxic, but its effect in other tissues is unknown. The aim of this work was to analyze the genotoxic potential of aluminum sulfate in kidney tissue of the fish Rhamdia quelen after trophic contamination for 60 days. Sixty four fish were subdivided into the following groups: negative control, 5 mg, 50 mg and 500 mg of aluminum sulfate per kg of fish. Samples of the posterior kidney were taken and prepared to obtain mitotic metaphase, as well as the comet assay. The three types of chromosomal abnormalities (CA) found were categorized as chromatid breaks, decondensation of telomeric region, and early separation of sister chromatids. The tests for CA showed that the 5 mg/kg and 50 mg/kg doses of aluminum sulfate had genotoxic potential. Under these treatments, early separation of the sister chromatids was observed more frequently and decondensation of the telomeric region tended to increase in frequency. We suggest that structural changes in the proteins involved in DNA compaction may have led to the decondensation of the telomeric region, making the DNA susceptible to breaks. Moreover, early separation of the sister chromatids may have occurred due to changes in the mobility of chromosomes or proteins that keep the sister chromatids together. The comet assay confirmed the genotoxicity of aluminum sulfate in the kidney tissue of Rhamdia quelen at the three doses of exposure.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000400499
Editor: | Sociedade Brasileira de Genética
Relação: | 10.1590/S1415-475738420140327
Formato: | text/html
Fonte: | Genetics and Molecular Biology  v.38 n.4 2015
Fonte: | info:eu-repo/semantics/openAccess