Data/hora: 02/06/2024 03:31:53

Provedor de dados: 	Genetics and Molecular Biology	
País: 	Brazil	
Título: 	Association of a PIT1 gene polymorphism with growth hormone mRNA levels in pig pituitary glands	
Autores: 	Franco,Maurício Machaim; Antunes,Robson Carlos; Oliveira,Katiana Mello de; Pereira,Cícero Donizete; Biase,Fernando Henrique; Nunes,Francis de Morais Franco; Goulart,Luiz Ricardo.	
Data: 	2005-03-01	
Ano: 	2005	
Palavras-chave: 	PIT1 gene; Growth hormone mRNA; Semi-quantitative RT-PCR; Pig.	
Resumo: 	Fourty-six non-castrated, halothane-free, male Landrace pigs were genotyped by PCR-RFLP for the Rsa I polymorphism in the PIT1 gene and classified into AA and AB genotypes. Total RNA was extracted from the pituitaries and the relative quantities of growth hormone (GH) mRNA were determined by semi-quantitative RT-PCR. Pigs with the AB genotype had higher levels of GH mRNA than those with the AA genotype (p = 0.034; Kruskal-Wallis test). This result suggests that the Rsa I polymorphism may be involved in Pit-1 protein expression or function, which in turn may influence GH transcription and expression. Thus, the Rsa I PIT1 gene polymorphism in this pig line may be used as a molecular marker to identify higher GH expression and possibly select for carcass and performance traits affected by GH.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000100003	
Editor: 	Sociedade Brasileira de Genética	
Relação: 	10.1590/S1415-47572005000100003	
Formato: 	text/html	
Fonte: 	Genetics and Molecular Biology  v.28 n.1 2005	
Fonte: 	info:eu-repo/semantics/openAccess