Data/hora: 28/05/2024 16:37:42

Provedor de dados: | Genetics and Molecular Biology
País: | Brazil
Título: | Effect of soybean ureases on seed germination and plant development
Autores: | Rechenmacher,Ciliana; Wiebke-Strohm,Beatriz; Oliveira-Busatto,Luisa A. de; Polacco,Joseph C.; Carlini,Célia R.; Bodanese-Zanettini,Maria H.
Data: | 2017-01-01
Ano: | 2017
Palavras-chave: | Plant development; Functional study; Transgenic plants; Mutants; Urease.
Resumo: | Abstract Urease catalyzes the hydrolysis of urea to ammonia and carbon dioxide. The ammonia (nitrogen (N) product of urease activity) is incorporated into organic compounds. Thus, urease is involved in N remobilization, as well as in primary N assimilation. Two urease isoforms have been described for soybean: the embryo-specific, encoded by the Eu1 gene, and the ubiquitous urease, encoded by Eu4. A third urease-encoding gene was recently identified, designated Eu5, which encodes the putative protein product SBU-III. The present study aimed to evaluate the contribution of soybean ureases to seed germination and plant development. Analyses were performed using Eu1/Eu4/Eu5-co-suppressed transgenic plants and mutants of the Eu1 and Eu4 urease structural genes, as well as a urease-null mutant (eu3-a) that activates neither the ubiquitous nor embryo-specific ureases. The co-suppressed plants presented a developmental delay during the first month after germination; shoots and roots were significantly smaller and lighter. Slower development was observed for the double eu1-a/eu4-a mutant and the eu3-a single mutant. The N content in transgenic plants was significantly lower than in non-transgenic plants. Among the mutants, eu3-a presented the lowest and eu1-a the highest N content. Altogether, these results indicate that increased ureolytic activity plays an important role in plant development.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000200209
Editor: | Sociedade Brasileira de Genética
Relação: | 10.1590/1678-4685-gmb-2016-0107
Formato: | text/html
Fonte: | Genetics and Molecular Biology  v.40 n.1 suppl.1 2017
Fonte: | info:eu-repo/semantics/openAccess