Data/hora: 10/06/2024 17:36:51

Provedor de dados: | 74
País: | Brazil
Título: | Insights into the Melipona scutellaris (Hymenoptera, Apidae, Meliponini) fat body transcriptome
Autores: | Sousa,Cristina Soares de; Serrão,José Eduardo; Bonetti,Ana Maria; Amaral,Isabel Marques Rodrigues; Kerr,Warwick Estevam; Maranhão,Andréa Queiroz; Ueira-Vieira,Carlos.
Data: | 2013-01-01
Ano: | 2013
Palavras-chave: | Fat body; Gene expression; Melipona; Stingless bees; Transcriptome.
Resumo: | The insect fat body is a multifunctional organ analogous to the vertebrate liver. The fat body is involved in the metabolism of juvenile hormone, regulation of environmental stress, production of immunity regulator-like proteins in cells and protein storage. However, very little is known about the molecular mechanisms involved in fat body physiology in stingless bees. In this study, we analyzed the transcriptome of the fat body from the stingless bee Melipona scutellaris. In silico analysis of a set of cDNA library sequences yielded 1728 expressed sequence tags (ESTs) and 997 high-quality sequences that were assembled into 29 contigs and 117 singlets. The BLAST X tool showed that 86% of the ESTs shared similarity with Apis mellifera (honeybee) genes. The M. scutellaris fat body ESTs encoded proteins with roles in numerous physiological processes, including anti-oxidation, phosphorylation, metabolism, detoxification, transmembrane transport, intracellular transport, cell proliferation, protein hydrolysis and protein synthesis. This is the first report to describe a transcriptomic analysis of specific organs of M. scutellaris. Our findings provide new insights into the physiological role of the fat body in stingless bees.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000200022
Editor: | Sociedade Brasileira de Genética
Relação: | 10.1590/S1415-47572013000200022
Formato: | text/html
Fonte: | Genetics and Molecular Biology  v.36 n.2 2013
Fonte: | info:eu-repo/semantics/openAccess