Data/hora: 10/06/2024 03:45:09

Provedor de dados: | Genetics and Molecular Biology
País: | Brazil
Título: | Patterns of ribosomal DNA distribution in hylid frogs from the  Hypsiboas faber  and  H. semilineatus  species groups
Autores: | Nunes,Rosana dos Reis Abrante; Fagundes,Valéria.
Data: | 2008-12-01
Ano: | 2008
Palavras-chave: | Hylids; Hypsiboas; Karyotypes; FISH; Ag-NORs; Hypsiboas semilineatus; H. faber.
Resumo: | We performed a comparative analysis of the nucleolus organizer region (NOR) distribution in the karyotypes of hylid frogs from two different taxonomic groups,  Hypsiboas faber  and  H. semilineatus.  Silver nitrate staining of NORs (Ag-NORs) and fluorescence  in situ  hybridization (FISH) with a rDNA probe were used to investigate the chromosomal location of rDNA loci in two species. The karyotype of  H. semilineatus  and the Ag-NORs distribution of the four species are presented herein for the first time. After conventional staining, the four species presented very similar karyotypes with 2n = 24, but Ag-NORs analyses revealed species-specific characteristics.  H. albomarginatus, H. faber  and  H. semilineatus  had one pair of interstitial Ag-NORs in the short arm of pairs 2, 11, and in the long arm of pair 7, respectively.  H. pardalis  presented telomeric NORs in the long arm of pair 11. Ag-NORs were heteromorphic in three of the species ( H.  pardalis ,  H. semilineatus  and  H. albomarginatus ) and FISH confirmed the differential activity of rDNA genes in  H. semilineatus . In the present study, 2n = 24 karyotypes could be distinguished by their Ag-NORs distribution. Our results further the knowledge about the cytogenetics of hylids from Brazil.
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000500028
Editor: | Sociedade Brasileira de Genética
Relação: | 10.1590/S1415-47572008005000024
Formato: | text/html
Fonte: | Genetics and Molecular Biology  v.31 n.4 2008
Fonte: | info:eu-repo/semantics/openAccess