Data/hora: 04/06/2024 15:57:35

Provedor de dados: 	74	
País: 	Brazil	
Título: 	Comparative study on the use of specific and heterologous microsatellite primers in the stingless bees Melipona rufiventris and M. mondury (Hymenoptera, Apidae)	
Autores: 	Lopes,Denilce Meneses; Campos,Lúcio Antônio de Oliveira; Salomão,Tânia Maria Fernandes; Tavares,Mara Garcia.	
Data: 	2010-01-01	
Ano: 	2010	
Palavras-chave: 	Melipona; Stingless bees; Microsatellite; Transferability.	
Resumo: 	Due to their high degree of polymorphism, microsatellites are considered useful tools for studying population genetics. Nevertheless, studies of genetic diversity in stingless bees by means of these primers have revealed a low level of polymorphism, possibly the consequence of the heterologous primers used, since in most cases these were not specifically designed for the species under consideration. Herein we compared the number of polymorphic loci and alleles per locus, as well as observed heterozygosity in Melipona rufiventris and M. mondury populations, using specific and heterologous primers. The use of specific primers placed in evidence the greater frequency of polymorphic loci and alleles per locus, besides an expressive increase in observed heterozygosity in M. rufiventris and M. mondury, thereby reinforcing the idea that populational studies should be undertaken by preferably using species-specific microsatellite primers.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000200030	
Editor: 	Sociedade Brasileira de Genética	
Relação: 	10.1590/S1415-47572010005000017	
Formato: 	text/html	
Fonte: 	Genetics and Molecular Biology  v.33 n.2 2010	
Fonte: 	info:eu-repo/semantics/openAccess